NTISthis.com

Evidence Guide: MSL975029 - Perform histological tests

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL975029 - Perform histological tests

What evidence can you provide to prove your understanding of each of the following citeria?

Process tissue

  1. Select processor program for routine and non-routine samples
  2. Perform equipment pre-use checks on the tissue processor to ensure sample integrity is maintained
Select processor program for routine and non-routine samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform equipment pre-use checks on the tissue processor to ensure sample integrity is maintained

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue

  1. Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load
  2. Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy
  3. Check tissue for complete processing prior to embedding
  4. Apply procedures to prevent cross-contamination between tissues
  5. Inspect blocks, reject and re-embed items that do not meet quality control standards
Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check tissue for complete processing prior to embedding

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect blocks, reject and re-embed items that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut tissue sections

  1. Check the flotation bath is ready and satisfactory for use
  2. Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch
  3. Secure block in microtome following specified safety directions
  4. Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts
  5. Cut thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing
  6. Float sections onto water bath to flatten tissues
  7. Pick up sections onto microscope slides ensuring patient identification on slides matches that on the block
  8. Compare the blocks and sections ensuring the tissue on slides matches that in the blocks
  9. Apply procedures to prevent cross-contamination between patient tissues
  10. Identify, troubleshoot and resolve common section quality control issues
  11. Inspect sections and reject any that do not meet quality control standards
Check the flotation bath is ready and satisfactory for use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Secure block in microtome following specified safety directions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Float sections onto water bath to flatten tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Pick up sections onto microscope slides ensuring patient identification on slides matches that on the block

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between patient tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify, troubleshoot and resolve common section quality control issues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect sections and reject any that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain tissue sections

  1. Prepare labile reagents for immediate use
  2. Select reagents for specified technique, ensuring reagent sequence matches standard procedure
  3. Stain sections according to method using the required quality control section and accommodating any authorised variations
  4. Mount slides using medium compatible with staining technique ensuring no bubbles
  5. Examine control sections microscopically to ensure expected staining outcomes are achieved and procedural artefacts are detected
  6. Collate and cross-check slides, blocks and test request forms to ensure requirements have been met
  7. Attach permanent label with case, specimen and stain details as required by workplace
  8. Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain
  9. Approve slides and release to pathologist
Prepare labile reagents for immediate use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select reagents for specified technique, ensuring reagent sequence matches standard procedure

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain sections according to method using the required quality control section and accommodating any authorised variations

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Mount slides using medium compatible with staining technique ensuring no bubbles

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine control sections microscopically to ensure expected staining outcomes are achieved and procedural artefacts are detected

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Collate and cross-check slides, blocks and test request forms to ensure requirements have been met

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Attach permanent label with case, specimen and stain details as required by workplace

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Approve slides and release to pathologist

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Process specimens and associated request forms

  1. Identify specimens and request forms that do not comply with minimum industry requirements for labelling, identification and test requests
Identify specimens and request forms that do not comply with minimum industry requirements for labelling, identification and test requests

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record any discrepancies and indicate what action is required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Log acceptable specimens into a laboratory information management system (LIMS) accurately and efficiently, applying required document tracking mechanisms

  1. Select processor program for routine and non-routine samples
Select processor program for routine and non-routine samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform equipment pre-use checks on the tissue processor to ensure sample integrity is maintained

  1. Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load
Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check tissue for complete processing prior to embedding

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect blocks, reject and re-embed items that do not meet quality control standards

  1. Check the flotation bath is ready and satisfactory for use
Check the flotation bath is ready and satisfactory for use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Secure block in microtome following specified safety directions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Float sections onto water bath to flatten tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Pick up sections onto microscope slides ensuring patient identification on slides matches that on the block

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between patient tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify, troubleshoot and resolve common section quality control issues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect sections and reject any that do not meet quality control standards

  1. Prepare labile reagents for immediate use
Prepare labile reagents for immediate use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select reagents for specified technique, ensuring reagent sequence matches standard procedure

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain sections according to method using the required quality control section and accommodating any authorised variations

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Mount slides using medium compatible with staining technique ensuring no bubbles

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine control sections microscopically to ensure expected staining outcomes are achieved and procedural artefacts are detected

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Collate and cross-check slides, blocks and test request forms to ensure requirements have been met

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Attach permanent label with case, specimen and stain details as required by workplace

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Approve slides and release to pathologist

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain laboratory records

  1. Complete control stain quality assessment forms to report quality control outcomes
Complete control stain quality assessment forms to report quality control outcomes

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

File and store samples to facilitate efficient retrieval as required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain instrument logs as required by accreditation checks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge